报告题目：Fluorescent organic nanoparticles and advanced fluorescence imaging techniques: design and applications

报告人：Yves Mély

Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS，Université de Strasbourg,   Faculté de armacie, 74,  Route du Rhin, 67401 ILLKIRCH Cedex, France. Yves.mely@unistra.fr

报告时间：10月15日上午9:30

报告地点：无机超分子楼一楼圆形报告厅

报告摘要：

The talk will be divided in two parts. The first part will be devoted to the design and applications of  fluorescent organic nanoparticles (NPs) with high brightness. We notably designed stable nano-emulsion droplets encapsulating lipophilic fluorescent derivatives at exceptionally high concentrations. These highly fluorescent nano-droplets were used to image the blood flow in zebrafishs. We also designed fluorescent polymer NPs brighter than QDs and exhibiting photo-induced reversible on/off fluorescence switching. This collective switching of hundreds of dyes was related to ultrafast excitation energy transfer and could be used for super-resolution imaging. Last, we designed efficiently loaded and stable sub-20-nm polymer particles that preserved the capacity to encapsulate large amounts of ionic dyes, generating NPs 10-fold brighter than QDs of the same size. The second part will deal with advanced fluorescence microscopy techniques that were implemented in the laboratory. Quantitative imaging techniques as well as high resolution techniques afford unprecedented information on the dynamics and interactions of proteins in live cells. The applications of these techniques will be illustrated with a protein of the Human immunodeficiency virus type 1 (HIV-1).

报告人简介：

Diplomas and career

Doctor in Pharmacy, 1984, Doctor in Biophysics, University L. Pasteur, Strasbourg, 1988.

Military service 1988-89 in Fribourg. Habilitation (Habilitation à diriger des recherches), 1996.<span "="" style="font-size:10.0pt;font-family:;">

Professor Assistant in Biophysics, Faculty of Pharmacy, University of Strasbourg, 1989.

Post doctoral training (1997) with a von Humboldt fellowship in the Max Planck Institute, Frankfurt

Professor of Biophysics, Faculty of Pharmacy, University of Strasbourg, since 1998. Promoted PRCE2 in 2013.

Research interests

Head of a team of 20 senior researchers and 30 students, my research activity can be divided in two research axis. 1) Development of cutting-edge fluorescence techniques (quantitative two-photon microscopy combining FLIM and FCS, single molecule spectroscopy, high resolution microscopy such as dSTORM/PALM, STED and AFM/fluorescence microscopy) and innovative environment-sensitive fluorescence probes. 2) Application of these techniques and probes to monitor biomolecular interactions, mainly with viral proteins.

Scientific animation

-  Since 2009, leader of the team “Biophotonics of the molecular and cellular interactions”.

- Since 2009, head of the Laboratory of Biophotonics and Pharmacology, UMR 7213 CNRS.

- From 2003-08, co-director of the French network GDR 2588 CNRS “Functional microscopy of the living”.

- Since 2011, chair of the conference series “Methods and Applications in Fluorescence”.

Publications and conferences

250 international publications (>6500 citations, H =47), ~ 100 invited conferences, > 250 posters in national and international conferences. Direction of 28 PhD thesis (22 defended and 6 in progress). Five of them got a PhD award.

Most productive scientist in HIV-1 nucleocapsid protein and most productive French scientist in fluorescence within the last 5 years

Awards

- Adrerus thesis prize in 1990.

- Research and doctoral directory bonus from 1993-2009. Excellence research bonus since 2009.

- Honorary Doctor of the National Taras Shevchenko University of Kiev, November 7th, 2011.

National committees

- Member of the national committee of the Universities (39th section) from 1999 to 2004, and (85th section) from  2009 to 2012.

- Member of the National Committee of the CNRS (30th section) from 2009 to 2012 and (28th section) since 2012.

- Member of the scientific committee of the ? Ligue contre le Cancer, région Alsace ?  2002-05.

- Member of a committee from the National Agency against AIDS, 2004-08;

- Member of numerous selection committees for professor-assistant and professor positions, since 1993.

- Member of ANR Blanc committee, SVSE 5 - Physique, Chimie du vivant et innovations biotechnologiques, 2013.

Editorial activities

- Editor in chief of Methods and Applications in Fluorescence, since 2012.

- Editor of The protein journal, Open Journal of Biophysics, ISRN Biochemistry, Conference papers in biology, Journal of Viruses, Biochemical Compounds

Organization of conferences

- Chair of the organizing committee of the 4th international retroviral NC symposium, Illkirch, 14-17 September 2003.

-   Chair of the organization committee of the Methods and Applications in Fluorescence MAF12, September 11-14, 2011.

-  Co-chair of the conference ? Fluorescent Biomolecules and their Building Blocks Design and Applications (FB3) ?, University of California, San Diego, La Jolla, CA, USA, August 6th – August 9th, 2014